Does anyone have any suggestions on what could be used. When using scbh in reductive amination, the ph must be maintained in the range of 68. Lane marker sample buffer, nonreducing, 5x, 5ml, 0. Show this safety data sheet to the doctor in attendance. Add 2aa and sodium cyanoborohydride mixes to each sample add 50 l of the 2aa dye followed by 50 l of sodium cyanoborohydride to each of the samples and controls. Carbonyl groups such as aldehydes, ketones, and glyox. Download msds pdf search msds currently available for download bioworld offers sodium cyanoborohydride for your research at low price. Sodium cyanoborohydride reagent grade, 95% sigmaaldrich.
Wear appropriate protective eyeglasses or chemical safety goggles as described by oshas eye and face protection regulations in 29 cfr 1910. Reason for citation sodium borohydride is on the hazardous substance list. Pierce coimmunoprecipitation coip kit thermo fisher scientific. Sds of sodium cyanoborohydride, safety data sheets, cas. Eye contact in the case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Do not use mouthtomouth method if victim ingested or inhaled the substance. Apr 01, 1982 reductive methylation of protein amino groups with formaldehyde and sodium cyanoborohydride is shown to give up to 25% yield of ncyanomethyl ch2cn product.
Sodium cyanoborohydride is preferred as a reducing agent over sodium borohydride as the latter will also reduce the reactive aldehydes to hydroxyls at the same time as reducing the schiffs bases. For the love of physics walter lewin may 16, 2011 duration. Pa 800 plus pharmaceutical analysis system carbohydrate. Structural integrity no detectable sodium cyanoborohydride, sodium cyanotrihydroborate, molecular formula. Sodium borohydride is widely used for the reduction of carbonylcontaining compounds to alcohols.
Important if the denatured protein precipitates, discard the sample and restart this process. W336 red sodium cyanoborohydride t, f w336 ox sodium periodate f w336 acid cabinet conc. The 2aa labeling kit contains two sets of the following reagents 15 samples per kit supplied in glass ampoules sealed under pure nitrogen. Sodium cyanoborohydride is preferred as a reducing agent over sodium borohydride as the latter will also reduce the reactive aldehydes to hydroxyls at the same time as reducing the schiff s bases.
Sodium triacetoxyborohydride, 97%, acros organics fisher. Your new online msds binder is a place for you to store the material safety data sheets you need to deploy. However, we make no warranty of merchantability or any other warranty. The entry for will lead you to the original literature which details the synthesis of sodium cyanoborohydride and also the typical procedures used in reductive amination or other selective reductions.
Suitable samples any purified glycans with free reducing termini can be labeled. Our msds database is an easy to use, do it yourself tool, and best of all. Sodium cyanoborohydride mda reaction conditions, hive. Purification of sodium cyanoborohydride 2 sodium cyanoborohydride 10g is dissolved in thf 80ml and 1 m methanolic hydrochloric acid is added until ph reaches 9. Sodium cyanoborohydride is a very hygroscopic material and contact with water or moist air should be avoided. Safety data sheet creation date 29jan2010 revision date 17jan2018 revision number 4 1. Sodium cyanoborohydride solution american elements. Sodium cyanoborohydride can be made by reacting equimolar quantities of sodium borohydride and hydrogen cyanide in thf. The submitter has found that use of sodium borohydride instead of sodium cyanoborohydride in the present procedure results in the almost exclusive formation of cyclohexanol with less than 3% of basic material.
Biotage mpcyanoborohydride is a macroporous polymer supported cyanoborohydride, which is a solidsupported equivalent of tetraalkylammonium cyanoborohydride. It has been tested as pretreatment for pulping of wood, but is too costly to be commercialized. Synthesis of nabh3 cn and related compounds thiokol. It is widely used in organic synthesis for the reduction of imines. The solution is then poured with stirring into dioxan 250ml. The bound cyanoborohydride can be utilized as a versatile reducing agent for the reductive amination of carbonyl compounds and reduction of imines. Sodium cyanoborohydride revision date 19january2018 eye contact in the case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Sodium cyanoborohydride msds material safety data sheet or sds, coa and coq, dossiers. Add 2750 l of ltnbm01 solution to the sodium cyanoborohydride vial and ensure it dissolves to completion. Read the safety data sheet for 1 m sodium cyanoborohydride. Firstaid measures general advice immediate medical attention is required. Buy sodium cyanoborohydride cas 25895607, a highly selective reducing agent for reductive amination, from santa cruz.
Previously 1, we demonstrated that sodium borohydride can be also successfully employed for the reductive amination of carbonyl compounds by inorganic acid salts of ammonia and primary and secondary amines. Sodium cyanoborohydride reagent grade american elements. The precipitate is collected and stirred for 2 h in ethyl acetate 250ml. Why sodium cyanoborohydride reactivity depends on ph. Please read the material safety data sheets msds for all chemicals used see appendix 2. The cyanoborohydride offers five times milder reduction compared to borohydride in the reductive amination process, reducing the schiff s. Safety data sheet according to federal register vol. It is used for bleaching wood pulp, as a blowing agent for plastics, and as a reducing agent for aldehydes and ketones. Sodium borohydride cas 16940662 106371 merck millipore. Since the reaction rate for the reduction of iminium ions is much faster than for ketones or even aldehydes, the reductive amination can be carried out as a onepot procedure by introducing the reducing agent into a mixture of the amine and carbonyl compound.
Contact can severely irritate and burn the skin and eyes with possible eye damage. According to the material safety data sheet msds special precautions must be taken when working with the chemical described above. Tm 2aa 2aminobenzoic acid monosaccharide release and. Pdf download buy article permissions and reprints all articles of this category. Skin contact wash off immediately with plenty of water for at least 15 minutes. Left over reagent which contains sodium cyanoborohydride has to be disposed separately in a clearly marked bottle due to its toxicity see msds. Sodium cyanoborohydride, sodium cyanotrihydroborate. Jul 14, 2011 i need to find a method to analyse the clearance of sodium cyanoborohydride throughout a process we believe it has been cleared but we are strugglinf to find an assay that will show this with high sensitivity. View product specific information, msds, references and buying faq.
Sodium borohydride revision date 17jan2018 attention is required. Breathing sodium borohydride can irritate the lungs causing coughing andor shortness of breath. Samples of the sodium cyanoborohydride prepared in examples 1114 and a sample of sodium cyanoborohydride prepared in accordance with the general teachings of u. To ensure high yield of labeled glycans, it may be necessary, for some samples, to increase the concentrations of the reducing and labeling reagents. Sodium tetrahydroborate powder recommended use laboratory chemicals. Sodium borohydride msds material safety data sheet or sds, coa and coq, dossiers. Msds contains all of the information required by those regulations. Lane marker sample buffer, nonreducing, 5x, 5 ml, 0. Detection method for sodium cyanoborohydride organic. I need to find a method to analyse the clearance of sodium cyanoborohydride throughout a process we believe it has been cleared but we are strugglinf to find an assay that will show this with high sensitivity.
This white solid, usually encountered as a powder, is a reducing agent that finds application in chemistry, both in the laboratory and on an industrial scale. Identification sodium borohydride is a white, odorless powder or pellet. Canadian ingredient disclosure list section 16 additional information msds creation date. Sodium cyanoborohydride is the chemical compound with the formula nabh3cn. Sodium cyanoborohydride 10g is dissolved in thf 80ml and 1 m methanolic hydrochloric acid is added until ph reaches 9. Examples include dna purification procedures, preservation of biological material, and as a solvent in organic synthesis. Sigmaaldrich material safety data sheet date printed. Enter what general purpose this chemical will be having in this laboratory. The cyanoborohydride offers five times milder reduction. It can be prepared either by treating sodium cyanide with borane, or by reacting sodium borohydride with mercuryii cyanide. Sodium borohydride can affect you when breathed in and by passing through your skin. Uses advised against not for food, drug, pesticide or biocidal product use details of the supplier of the. The bound cyanoborohydride can be utilized as a versatile reducing agent for the reductive amination of carbonyl compounds and reduction of. Sodium cyanoborohydride cas 25895607 818053 emd millipore.
Biotage mpcyanoborohydride is a macroporous polymer supported cyanoborohydride. Find msds or sds, a coa, data sheets and more information. Sds of sodium cyanoborohydride, safety data sheets, cas 25895. Sodium cyanoborohydride is especially suitable for reductive aminations. Department of chemistry, university of minnesota, minneapolis, minnesota 55455. Identification product name sodium borohydride cat no. Add 2aa and sodium cyanoborohydride mixes to each sample. Sodium borohydride, also known as sodium tetrahydridoborate and sodium tetrahydroborate, is an inorganic compound with the formula na bh 4. How can i handle and dispose sodium cyanoborohydride.
Sodium cyanoborohydride cas 25895607 msds guidechem. In normal use conditions and in its original form, the product itself does not involve any other risk for health and the environment. This solution is filtered, heated to reflux on a steam bath and then dioxan 150ml. There is no warranty of accuracy or completeness of any information contained herein. The cyanoborohydride offers five times milder reduction compared to borohydride in. Ludgertag 2aa 2aminobenzoic acid glycan labeling kit. Very high exposure to sodium borohydride may affect the nervous system. Reductive amination of mdp2p using sodium cyanoborohydride. Amount of sample from 25 pmol up to 25 nmol glycans per sample. Sodium cyanoborohydride cas 25895607 scbt santa cruz. Sodium cyanoborohydride is the chemical compound with the formula na b h 3 cn. And it is usually used as a mild reducing agent which converts imines to amines. I am considering using sodium cyanoborohydride in a reductive nalkylation of a polysaccharide, and want to know how to handle it safely and dispose of the nitrile byproduct.
Filtration followed by vacuum removal of thf gives white solid sodium cyanoborohydride. Number of samples typically, up to 15 separate analytical samples per set of labeling reagents. Reductive methylation of protein amino groups with formaldehyde and sodium cyanoborohydride is shown to give up to 25% yield of ncyanomethyl ch2cn product. It is a colourless salt, but commercial samples can appear tan. Safety data sheet 112030 sodium triacetoxyborohydride.
After the addition is complete, the suspension is stirred for 30 minutes. Sigmaaldrich offers a number of sodium cyanoborohydride products. Wear protective glovesprotective clothingeye protectionface protection. Immediately flush eyes with running water for at least 15 minutes, keeping eyelids open. Firstly a summary of the preparation and properties of sodium cyanoborohydride is given. Allowing the material to come into contact with water or moist air may decompose the material and could lead to a violent reaction or explosion due to the release of hydrogen gas. Acid destabilizes the sodium cyanoborohydride which results in an activated state for kinetics and equilibrium. Articles of sodium cyanoborohydride are included as well. Specifically, compared with the small molecule sodium cyanoborohydride, it is reported that. Other companies are charging thousands of dollars to set up accounts and give you access to their msds online database. Thermo scientific aminolink reductant sodium cyanoborohydride promotes the formation of stable bonds between aldehyde and aminecontaining molecules, enabling efficient labeling, conjugation, and immobilization of proteins and other molecules.